How to Reconstitute Peptide Vials

A peptide vial is a clear glass vial with an HDPE frit, snap cap with septum and a silicone seal. These vials are ideal for the storage of lyophilized peptides in your laboratory. Peptide solutions have a very limited shelf-life and can be subject to bacterial degradation over time. For this reason, peptides in solution are usually shipped as lyophilized powders and require reconstitution prior to use. It is recommended to aliquot any peptide solutions that are not being used within four weeks and store them at -20°C or lower, as opposed to long-term storage in a refrigerator, which can cause chemical degradation and a loss of potency over time.

To properly prepare and handle your peptides, you will need a few essential items including peptide, sterile water, a syringe or pipette and a sterile container or vial to hold the reconstituted peptide. The amount of water required to reconstitute your peptide will depend on the concentration of the peptide and should be detailed in the product description or instructions that come with your peptides.

Once you have gathered all the items needed, it is time to begin the reconstitution process. The first step is to thoroughly clean the rubber area of the peptide vial with an alcohol swab. Next, you will need to withdraw 1mL (milliliters) of bacteriostatic water into the peptide vial and gently swirl until all of the powder dissolves. Finally, you will transfer the peptide solution to the sterile container or syringe and remove the needle, which should now contain your reconstituted peptides uk

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